1x mem vitamin solution Search Results


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1x Mem Vitamin Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher rpmi4spy medium
The chemically defined medium <t>RPMI4Spy</t> enables fluorescence measurements in plate readers. (A) Composition of the RPMI4Spy growth medium. <t>RPMI1640</t> (with glutamine and without phenol red) forms the base of this medium. (B) Growth of the S. pyogenes SF370 wildtype shown as OD 620nm in THY medium compared to RPMI4Spy. (C) Top row: Growth (OD 620nm ) of the mNeongreen reporter strain (green) and the control strain harboring mNeongreen without any promoter in THY medium (left), chemically defined medium (CDM) (center) and the RPMI4Spy medium (right). Middle row: Relative fluorescence units (RFUs) measured for medium only (black), the mNeongreen reporter strain (green) and the control strain harboring mNeongreen without any promoter (grey) in THY medium (left), CDM (center) and the RPMI4Spy medium (right). Bottom row: Relative fluorescence units calculated and normalized by optical density (RFU/OD 620nm ) measured for the mNeongreen reporter strain (green) and the control strain harboring mNeongreen without any promoter in THY medium (left), CDM (center) and the RPMI4Spy medium (right). Experiments were performed in biological triplicates and each measurement in technical duplicates.
Rpmi4spy Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher 1x hl5 medium
The chemically defined medium <t>RPMI4Spy</t> enables fluorescence measurements in plate readers. (A) Composition of the RPMI4Spy growth medium. <t>RPMI1640</t> (with glutamine and without phenol red) forms the base of this medium. (B) Growth of the S. pyogenes SF370 wildtype shown as OD 620nm in THY medium compared to RPMI4Spy. (C) Top row: Growth (OD 620nm ) of the mNeongreen reporter strain (green) and the control strain harboring mNeongreen without any promoter in THY medium (left), chemically defined medium (CDM) (center) and the RPMI4Spy medium (right). Middle row: Relative fluorescence units (RFUs) measured for medium only (black), the mNeongreen reporter strain (green) and the control strain harboring mNeongreen without any promoter (grey) in THY medium (left), CDM (center) and the RPMI4Spy medium (right). Bottom row: Relative fluorescence units calculated and normalized by optical density (RFU/OD 620nm ) measured for the mNeongreen reporter strain (green) and the control strain harboring mNeongreen without any promoter in THY medium (left), CDM (center) and the RPMI4Spy medium (right). Experiments were performed in biological triplicates and each measurement in technical duplicates.
1x Hl5 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fisher Scientific 1x mem vitamins
The chemically defined medium <t>RPMI4Spy</t> enables fluorescence measurements in plate readers. (A) Composition of the RPMI4Spy growth medium. <t>RPMI1640</t> (with glutamine and without phenol red) forms the base of this medium. (B) Growth of the S. pyogenes SF370 wildtype shown as OD 620nm in THY medium compared to RPMI4Spy. (C) Top row: Growth (OD 620nm ) of the mNeongreen reporter strain (green) and the control strain harboring mNeongreen without any promoter in THY medium (left), chemically defined medium (CDM) (center) and the RPMI4Spy medium (right). Middle row: Relative fluorescence units (RFUs) measured for medium only (black), the mNeongreen reporter strain (green) and the control strain harboring mNeongreen without any promoter (grey) in THY medium (left), CDM (center) and the RPMI4Spy medium (right). Bottom row: Relative fluorescence units calculated and normalized by optical density (RFU/OD 620nm ) measured for the mNeongreen reporter strain (green) and the control strain harboring mNeongreen without any promoter in THY medium (left), CDM (center) and the RPMI4Spy medium (right). Experiments were performed in biological triplicates and each measurement in technical duplicates.
1x Mem Vitamins, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+mem+vitamin+solution/10__1021_slash_jm030545e-106-64-73?v=Fisher+Scientific
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Image Search Results


The chemically defined medium RPMI4Spy enables fluorescence measurements in plate readers. (A) Composition of the RPMI4Spy growth medium. RPMI1640 (with glutamine and without phenol red) forms the base of this medium. (B) Growth of the S. pyogenes SF370 wildtype shown as OD 620nm in THY medium compared to RPMI4Spy. (C) Top row: Growth (OD 620nm ) of the mNeongreen reporter strain (green) and the control strain harboring mNeongreen without any promoter in THY medium (left), chemically defined medium (CDM) (center) and the RPMI4Spy medium (right). Middle row: Relative fluorescence units (RFUs) measured for medium only (black), the mNeongreen reporter strain (green) and the control strain harboring mNeongreen without any promoter (grey) in THY medium (left), CDM (center) and the RPMI4Spy medium (right). Bottom row: Relative fluorescence units calculated and normalized by optical density (RFU/OD 620nm ) measured for the mNeongreen reporter strain (green) and the control strain harboring mNeongreen without any promoter in THY medium (left), CDM (center) and the RPMI4Spy medium (right). Experiments were performed in biological triplicates and each measurement in technical duplicates.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Expanding the genetic toolbox for the obligate human pathogen Streptococcus pyogenes

doi: 10.3389/fbioe.2024.1395659

Figure Lengend Snippet: The chemically defined medium RPMI4Spy enables fluorescence measurements in plate readers. (A) Composition of the RPMI4Spy growth medium. RPMI1640 (with glutamine and without phenol red) forms the base of this medium. (B) Growth of the S. pyogenes SF370 wildtype shown as OD 620nm in THY medium compared to RPMI4Spy. (C) Top row: Growth (OD 620nm ) of the mNeongreen reporter strain (green) and the control strain harboring mNeongreen without any promoter in THY medium (left), chemically defined medium (CDM) (center) and the RPMI4Spy medium (right). Middle row: Relative fluorescence units (RFUs) measured for medium only (black), the mNeongreen reporter strain (green) and the control strain harboring mNeongreen without any promoter (grey) in THY medium (left), CDM (center) and the RPMI4Spy medium (right). Bottom row: Relative fluorescence units calculated and normalized by optical density (RFU/OD 620nm ) measured for the mNeongreen reporter strain (green) and the control strain harboring mNeongreen without any promoter in THY medium (left), CDM (center) and the RPMI4Spy medium (right). Experiments were performed in biological triplicates and each measurement in technical duplicates.

Article Snippet: For fluorescence measurements, S. pyogenes was cultured in RPMI4Spy medium [Gibco™ RPMI1640 medium with glutamine and without phenol red, 1x Nucleobase mix (20 mg/L Guanine, 20 mg/L Adenine and 20 mg/L Uracil), 1x BME Vitamin solution, 1x RPMI Amino Acids solution, 7 g/L Glucose, 0.02 mM HEPES buffer (pH 7.4), 10 mg/L Niacinamide].

Techniques: Fluorescence, Control